The Tezuka Osamu Shakespeare Mangakan (Osamu Tezuka's Shakespeare Manga House) book features "Hamlet," "Othello," and "Jajauma Narashi" (The Taming of the Shrew) from Tezuka's Rainbow Parakeet manga; "Macbeth" from "Sannin no Uranaishi" (The Three Fortunetellers) in The Vampires; "Robio to Robiet" (Robio and Robiet) from a Romeo and Juliet-inspired section of Astro Boy; and "Venice no Shōnin" (The Merchant of Venice). The book marks the first time that color manuscripts of the manga have been published.

In addition to manga, the book features introductions to Shakespeare's works that inspired Tezuka's creations. The volume also includes columns written by Tezuka.