For me it is from Gunslinger Girl
Spoiler (mouse over to view)
Death of Angelica. It was very sad yet beautiful. The feelings were conveyed really good and this :
http://mangafox.me/manga/gunslinger_girl/v09/c048/19.html
The only scene that made me cry so far.
http://mangafox.me/manga/gunslinger_girl/v09/c048/19.html
The only scene that made me cry so far.