Is there a manga where the main character wants to remain a virgin? Or they are refraining from sex?
(Example of what I have already read:My Balls) Although there is some opportunities that make it hard on them? And maybe causes misunderstandings?

I don't care what genre, but I would prefer a comedy and a not a yaoi/shonen-ai.

________________

Bousou Shojo, I think this fits your bill quite well, link on the bottom.

http://www.mangaupdates.com/series.html?id=30773

Hey I've actually read that, is there any others?

________________

I believe Doubt!! would fit the description.

http://www.mangaupdates.com/series.html?id=1125

________________

Mysterious Girlfriend X
Chobits Although, can you really lose your virginity to a machine in the first place? Well, that and many other interesting questions are raised in this series.


________________
Sarcasm just doesn't work over the internet.