When Luffy awokes and realize that Ace is dead...

when hikaru finally realize that sai was gone after reading Honinbo Shusaku's kifu

Ace's Death; I read that 5 times and cried each time. And Luffy's realization of his death

when izumi let hikaru realize that sai lives on the way hikaru plays go

when hikaru dreamt about sai.. seeing sai's nostalgic smile, and specially when sai handed hikaru his (sai's) fan.. too bad the fan is only in the dream.. it will really be nice if its also in reality...

in Men's Kou, i was expecting a reunion of torn apart lovers who had disagreements or something. Typical shoujo manga stuff so basically was expecting that Chika and Maki get back together as we see Maki remembering her. And at the end of chapter 6 Maki says "chika your not in this world anymore", it toally crushed all my expectatons because chika was an awesome girl, and there was no reunion, just maki trying to live on without her, trying to forget about her but not being able to and from the book which he got from chika that he just recently found there was a letter circled on each page, and together it said "I like you" for the first time chika expressed her feelings but she was not alive anymore.

also Naruto, when Itachi dies and it gets revealed how much he had suffered and how much he really cared about that asshole Sasuke. There is more but well too much typing would be needed.

most of Nana scenes, especially nobu/hachiko ones at middle/end
and nana/ren ones though out the anime

One Piece:
-When the Strawhats got separated at Shabondy by Kuma
-When Ace died
-When Whitebeard died

DBZ:
-When Vegeta died on Namek

when Ace died.

when merry got burned, so very sad

The moment when he got stabbed and he died and both nunnaly and kallen/karren understood that it had all been planned out. SO SAD

when ussop challenge luffy and luffy decided to accept the challenge and beat ussop, even if it's against his decision.

after sogeking hit the government flag with a fire star, Robin declared she wanna live

when Iceberg narrates to Nami that Nico's only wish is to save her nakama

When Shin finally killed himself together with Mana...

when makiko said sorry to souchirou after sacrificing herself to revive maya.

in one piece where they part with merry, when chopper accidentally kills the doctor, when the crew gets broken up at sabaody.
But the saddest part to me of all time was WHEN ACE DIES. I was sobbing and was like :O cause i would neve had expected that development. Also the aftermath with Luffy totally breaking down and bawls @ the woman island (refusing to believe that ace died) was also very very heartbreaking.

When Euphemia goes crazy and dies. Also when Lelouch gets stabbed and Nunally is crying over her brother's body while everybody else is cheering.

lelouch will ever keep on living in my heart if i don´t see it

when kurosawa himself cries because he´s heartbroken